第二法（填充柱法）
色谱条件与系统适用性试验 用直径为0.18～0.25mm的二乙烯苯-乙基乙烯苯型高分子多孔小球作为载体；柱温125℃。理论板数按甲醇峰计算应不低于1500；甲醇峰、乙醇峰与内标物质各相邻色谱峰之间的分离度应符合规定。
校正因子测定 精密量取正丙醇1ml，置100ml量瓶中，用水溶解并稀释至刻度，摇匀，作为内标溶液。另精密量取甲醇1ml，置100ml量瓶中，用水稀释至刻度，摇匀，精密量取10ml，置100ml量瓶中，精密加入内标溶液10ml，用水稀释至刻度，摇匀，取1μl注入气相色谱仪，连续进样3～5次，测定峰面积，计算校正因子。
测定法 精密量取内标溶液1ml，置10ml量瓶中，加供试液至刻度，摇匀，作为供试品溶液，取lμl注入气相色谱仪，测定，即得。
除另有规定外，供试液含甲醇量不得过0.05% （ml/ml） 。
编号：20231007007
型号：HQ填充柱
规格：2m*1/8，60-80目
*高使用温度：200°C
Second method (filled column method)
Chromatographic conditions and system suitability tests were carried out using porous microspheres of divinylbenzene ethylenebenzene type polymers with a diameter of 0.18-0.25mm as carriers; The column temperature is 125 ℃. The number of theoretical plates calculated based on methanol peak should not be less than 1500; The resolution between the methanol peak, ethanol peak, and adjacent chromatographic peaks of the internal standard substance should comply with regulations.
Precision measurement of calibration factor: Take 1ml of n-propanol and place it in a 100ml volumetric flask. Dissolve and dilute with water to the mark, shake well, and use it as the internal standard solution. Accurately measure 1ml of methanol and place it in a 100ml volumetric flask. Dilute with water to the mark, shake well. Accurately measure 10ml and place it in a 100ml volumetric flask. Accurately add 10ml of internal standard solution and dilute with water to the mark, shake well, and take 1 μ Inject into the gas chromatograph, continuously inject 3-5 times, measure the peak area, and calculate the correction factor.
Precision measurement method: Take 1ml of the internal standard solution, place it in a 10ml volumetric flask, add the test solution to the mark, shake well, and use it as the test solution. Take l μ Inject into a gas chromatograph, measure, and obtain.
Unless otherwise specified, the methanol content of the test solution shall not exceed 0.05% (ml/ml).
No.: 20231007007
Model: HQ filled column
Specification: 2m * 1/8, 60-80 mesh
*High operating temperature: 200 ° C
滕州市浩瀚色谱仪器技术服务有限公司
电话：0632-5667636
传真：0632-5667636
手机：15562228838,13963221227
地址：山东滕州市平行路（商务部和技术部）
邮箱：wangxiaoying9@126.com
QQ:1404939462
联系人：王经理
网址：www.haohansepu.com