新型合成酚类抗氧化剂叔丁基对苯二酚(TBHQ)通过生产和消费进入环境，对人体健康和生态环境造成影响。目前，其安全性仍存在很大争议，而我国尚无食品中TBHQ的标准检测方法。基于“绿色化学”的环境保护理念，为从源头对其进行控制，满足当前对食品中TBHQ添加量进行检测监督的需求和进一步完善TBHQ的毒理学评价。
浩瀚色谱（山东）应用技术开发有限公司建立了定量检测食用植物油中TBHQ的方法，研究了TBHQ在大鼠血清中的代谢规律，运用血清药理学方法对TBHQ进行了毒性评价。取得的主要成果如下： (1)建立了能够快速、准确、灵敏地检测食用植物油中TBHQ的液相色谱/质谱联用法。该方法线性好(R<'2>=0.9990)，检出限低(0.3 mg/kg)，准确度高(回收率83.3～110.8％)，重现性好(日内相对标准偏差为2.5％，同问相对标准偏差为7.6％)，耗时短。
本标准规定了采用气相色谱、液相色谱测定叔丁基对苯二酚(TBHQ)的原理、试剂、仪器和设备、分析步骤、结果计算及精密度。
本标准适用于较低熔点的食用植物油中TBHQ含量的测定。本标准不适用于熔点高于35℃以上的食用植物油中TBHQ含量的测定。
本标准定量限：气相色谱法为0.001g/kg，液相色谱法为0.006g/kg。
本标准中气相色谱法为仲裁方法。
名称：玻璃柱
规格：3mm（内径）*3m
型号:2%OV-1/Chromosorb W AW DMCS 80-100目
应用：GB/T 21512-2008 食用植物油中叔丁基对苯二酚（TBHQ）的测定
适用于适用于岛津GC-14C，GC-2010，GC-2014，GC-2030等各种型号
Tert-butylhydroquinone (TBHQ), a new synthetic phenolic antioxidant, enters the environment through production and consumption, which has an impact on human health and ecological environment. At present, its safety is still controversial, and there is no standard test method for TBHQ in food in China. Based on the environmental protection concept of "green chemistry", in order to control it from the source, meet the current demand for detection and supervision of TBHQ addition in food and further improve the toxicological evaluation of TBHQ.
Haohan Chromatography (Shandong) Applied Technology Development Co., Ltd. has established a method for quantitative detection of TBHQ in edible vegetable oil, studied the metabolic rule of TBHQ in rat serum, and conducted toxicity evaluation of TBHQ using serum pharmacological methods. The main achievements are as follows: (1) A liquid chromatography/mass spectrometry method for the rapid, accurate and sensitive detection of TBHQ in edible vegetable oil was established. The method has good linearity (R<'2>=0.9990), low detection limit (0.3 mg/kg), high accuracy (recovery rate of 83.3~110.8%), good reproducibility (intra-day relative standard deviation of 2.5%, intra-day relative standard deviation of 7.6%) and short time.
This standard specifies the principle, reagents, instruments and equipment, analytical steps, result calculation and precision of determination of tert-butylhydroquinone (TBHQ) by gas chromatography and liquid chromatography.
This standard is applicable to the determination of TBHQ content in edible vegetable oil with lower melting point. This standard is not applicable to the determination of TBHQ content in edible vegetable oil with melting point higher than 35 ℃.
The quantitative limit of this standard: 0.001g/kg for gas chromatography and 0.006g/kg for liquid chromatography.
In this standard, gas chromatography is the arbitration method.
Name: glass column
Specification: 3mm (inner diameter) * 3m
Model: 2% OV-1/Chromosorb W AW DMCS 80-100 mesh
Application: GB/T 21512-2008 Determination of tert-butylhydroquinone (TBHQ) in edible vegetable oil
It is applicable to all kinds of Thermo Flight 1310 instruments
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